The local ambiguity is a very common condition in optical microscopy. The reason for this is that the surface of the sample is not flat enough, because the surface of the sample is not on a parfocal surface, so only the focus is clearly observed, but not Part of the same focus height will be blurred.

In the above situation, you can do the test piece inlay, or else you can only take the high number of the accompanying number, and cut the middle of the middle to clear the unclear edge. I don't know if your software has extended depth of field function, apply EFI depth of field extension function, rotate adjustment, capture when the picture is clear, then rotate and adjust, then shoot. Always take all the clear parts, apply this function, it is a very clear picture.

The test piece will not be too flat, you need the Z-axis depth of field function, you can also try a multi-focus microscope.

The above is the solution to the partial blurring of the optical microscope, I hope to help you! If you need related products, please call us.