In a fluorescence microscope, a sample emits energy when activated by light of a particular wavelength. Some substances, such as chlorophyll and some minerals, do this naturally, while others require the help of other chemical triggers.

Fluorescence microscopy usually consists of the following components:

- Light source, which can be a xenon or mercury lamp, LED or laser.
- an excitation filter that selects only the excitation wavelength from the source that is capable of exciting the sample.
- A dichroic beam splitter or mirror that transmits and reflects light as a function of wavelength.
- A transmit filter that ensures that only light emitted from the sample is transmitted by selecting light of different wavelengths.
- CCD camera, a device widely used to detect emitted light, usually connected to a computer screen displaying the generated image

The above is about the introduction of the relevant knowledge of fluorescence microscope, I hope to help friends who have this need! To learn more about fluorescence microscopy, stay tuned.